![](https://parts.igem.org/images/partbypart/icon_dna.png)
DNA
Part:BBa_J435371
Designed by: Open Bioeconomy Group: FreeGenes (2023-05-09)
EF_TZ
Strong triple terminator (UUCG_T7-TΦ , rnnBT1, T7-TΦ), defined as an EF part.
FLANKED 5' fusion site: GCTT, 3' fusion site: CGCT
Source organism: N/A, Target organism: Escherichia coli
ORG: E. coli Protein Expression Toolkit, Open Bioeconomy & The FreeGenes Project
URL: https://freegenes.github.io/genes/BBF10K_003477.html
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 7
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 7
Illegal NotI site found at 149 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 7
Illegal BamHI site found at 1
Illegal XhoI site found at 158 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 7
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 7
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |